Decontamination of enteric pathogens in soil ecosystems irrigated with low quality water for continuous irrigation practice
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Agricultural Microbiology, National research Centre, Egypt
Soils and Water Use Department, National research Centre, Egypt
Submission date: 2021-10-05
Final revision date: 2022-01-17
Acceptance date: 2022-04-02
Online publication date: 2022-04-02
Corresponding author
Hoda Kabary   

Agricultural Microbiology, National research Centre, 33 El-Buhouth st, Dokki,, 12661, Cairo, Egypt
Soil Sci. Ann., 2022, 73(1)147964
Recently, management of soil irrigated with low-quality water has become necessary to have a healthy crop. This research work aims to design the best management practices (BMP) to minimize drainage water hazards in the soil ecosystems. Based on the source of irrigation water, a column experiment was implemented on three soil samples that collected from three governorate in Egypt. The subsistence of enteric pathogens in soil irrigated with three types of low-quality water either sole sewage effluent (Giza) or a mix of drainage and industrial effluent (Kafr-el-sheikh) or drainage effluent (Sinai) was monitored periodically for 90 days. The trailed soils were divided as: non inoculated cultivated (C) or inoculated cultivated with sole phosphate dissolving bacteria (T1) or Acidithiobacillus sp. (T2) or with a combination of both microorganisms (T3). Three common hyperaccumulator plants (Brassica napus, Plantago psyllium, and Plantago major) were cultivated separately in cultivated, inoculated soil trials in comparison to non-cultivated, non-inoculated control treatments (NC). Results section illustrates the removal pattern of fecal coliforms and Salmonella sp. in the trailed soil ecosystems, in response to different treatments during 90 days of experimental monitoring. The trailed remediation amendments, either in the single or combined application, followed by phytoremediation with three different phytoremediation plants, exhibited a positive effect in diminishing pathogenic bacteria in the three tested soil ecosystems, yet at varying degrees. The study concludes that, applied mixture of all treatments represented by choice of Plantago psyllium (as best phytoremediator plant) and combination of two remeditative bacterial inoculums (Acidithiobacillus and phosphate dissolving bacteria) in contaminated soil was selected as the BMP among the other applied treatments.
APHA, American Public Health Association (1995) Standard Methods for the Examination of Water and Wastewater 19th ed., APHA, Washington.
Bhattacharyya, K.G., Sen Gupta, S., 2009. Adsorptive accumulation of Cd(II), Co (II), Cu(II), Pb(II) and Ni(II) ions from water onto kaolinite: Influence of acid activation. Adsorption Science & Technology 27(1), 47–68.
El-Korashy, S.A., Elwakeel, K.Z., Abd El-Hafeiz, A., 2016. Fabrication of bentonite/thiourea-formaldehyde composite material for Pb(II), Mn(VII) and Cr(VI) sorption: A combined basic study and industrial application. Journal of Cleaner Production 137, 40.
FAO, 2007. Handbook on Pressurizes Irrigation Techniques, 2nd edition, chapter 7, water quality for irrigation pp. 1-33.
Gerba, C., Bitton, G., 1984. Microbial pollutants: their survival and transport pattern to groundwater. In: Bitton, G. and Gerba, C.P. (eds) Groundwater pollution microbiology. Wiley, New York, 65-88.
Gutierrez-Gines, M. J., Alizadeh, H., Alderton, E., Ambrose, V., Meister, A., Robinson, B. H., Halford, S., Prosser, J. A., Horswell, J., 2021. Phytoremediation of microbial contamination in soil by New Zealand native plants. Applied Soil Ecology 167,104040.
Hassanain, N., Shaapan, R., Saber, M., Kabary, H., Zaghloul, A., 2021. Adverse Impacts of Water Pollution from Agriculture (Crops, Livestock, and Aquaculture) on Human Health, Environment, and Economic Activities. Egyptian Journal of Aquatic Biology & Fisheries 25(2), 1093–1116.
Housing & Building National research Center, 2004. The Egyptian Manual as Guidelines for Treated Waste Water Reuse in Agriculture. Ministry of Housing & Utilities and New Communities, Cairo.
Kabary, H., Hassanain, N., Saber, M., Zagloul, A., 2021a. Enumeration and Detection of Main Pathogenic Bacterial Genera in Rahawy and Bilbeis Agriculture Drains. Egyptian Journal of Aquatic Biology & Fisheries 25(5), 189–202.
Kabary, H., Saber, M., El-Ashry, S., Zaghloul, A., 2021b. An integrated chemo- bio- mineral technology for agricultural drainage water remediation, International Journal of Environmental Analytical Chemistry.
Liu, C., Xie, X., Zhao, W., Liu, N., Maraccini, P.A., Sassoubre, L.M., Boehm, Ab., Cui, Y., 2013a. Conducting nanosponge electroporation for affordable and high-efficiency disinfection of bacteria and viruses in water. Nano Letters 13, 4288–4293.
Ma, X., Zhou, W., Fu, Z., Cheng, Y., Min, M., Liu, Y., Zhang, Y., Chen, P., Ruan R., 2014. Effect of wastewater-borne bacteria on algal growth and nutrients removal in wastewater-based algae cultivation system. Bioresource Technology 167, 8–13.
Nygård, K., Lassen, J. Vold, L., Andersson, Y., Fisher, I., Löfdahl, S., Threlfall, J., Luzzi, I., Peters, T., Hampton, M., 2008. Outbreak of Salmonella Thompson infections linked to imported rucola lettuce. Foodborne Pathogens and Disease 5, 165–173.
Quinn, P., Carter, M., Markey, B., Donnelly, W., Leonard, F., 2002. Antimicrobial agents. Great Britain by MPG, Books Ltd.
Saber, M., 2007. Strategic prospective for reuse of sewage effluents in agriculture Proceeding of The Fourth ERD6 Conference, Faculty of Engineering, Shebin El-Kom, Center of Rural Development.
Saber, M., Abu-sedera, S., Matter, I. A., Zaghloul, A., 2015. Seasonal Variations in the Microbial Biomass and Pathogenic Bacteria in some Egyptian Sewage Effluents. Research Journal of Pharmaceutical, Biological and Chemical Sciences 6(4), 1709–1717.
Saber, M., Hoballah, E., Azza, Sh., Doaa, I.A., 2011. Microbial Decontamination of Enteric Pathogens in Sewaged Soils. Australian Journal of Basics and Applied Sciences 5(11), 1312–1320.
Saber, M., Hoballah, E., El-Ashry, S., Abouziena H.F., Zaghloul A. M., 2016. Bioremediation of Soil Irrigated with Sewage Effluent Benefitting New Kinetic Tactics Ponte Journal 72(3), 67–82.
Santamarı, A.J., Toranzos, G., 2002. Enteric pathogens and soil: a short review. International Microbiology 6(1), 5–9.
Staley, J.T., Bryant, M.P., Pfennig, N., Holt, J.G., 1989. “Acidithiobacillus,” in Bergey’s Manual of Systematic Bacteriology, 1st Ed., Vol. 3, ed. W. A. Wilkins (Baltimore, MD: Springer), 1842–1858.
Straub, T., Pepper, I., Gerba, C., 1992. Persistence of viruses in desert soils amended with anaerobically digested sewage sludge. Applied and Environmental Microbiology 58, 636–641.
Tabak, M., Lisowska, A., Filipek-Mazur, B., Antonkiewicz, J., 2020. The effect of amending soil with waste elemental sulfur on the availability of selected macroelements and heavy metals. Processes, 8, 1245.
Tyagi, V.K., Chopra, A.K., Kazmi, A.A., Kumar, A., 2006. Alternative microbial indicators of fecal pollution: current perspective. Iranian Journal of Environmental Health, Science and Engineering 3, 205–216.
WHO, 1989. Health Guidelines for the use of wastewater in agriculture and aquaculture. Technical report series no 778, WHO, Geneva.
WHO, 2006. Guidelines for the safe use of wastewater, excreta and grey water: Wastewater use in agriculture, vol 2. WHO Technical Report Series 778. World Health Organization, Geneva.
Zhao, Z., Jiang, G., Mao, R., 2014. Effects of particle sizes of rock phosphate on immobilizing heavy metals in lead zinc mine soils. Soil Science and Plant Nutrition 14, 258–266.
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