Effects of neem leaf extract on inorganic nitrogen transformation in sandy soil
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Faculty of Agricultural Technology, Plant Science Section, Sakon Nakhon Rajabhat University, Thailand
Faculty of Natural Resources, Department of Thai Traditional Medicine, Rajamangala University of Technology Isan, Thailand
Submission date: 2022-08-02
Final revision date: 2022-09-08
Acceptance date: 2022-10-26
Online publication date: 2022-10-26
Publication date: 2022-11-25
Corresponding author
Somchai Butnan   

Faculty of Agricultural Technology, Plant Science Section, Sakon Nakhon Rajabhat University, 47000, Sakon Nakhon, Thailand
Soil Sci. Ann., 2022, 73(4)156072
The inhibitory effects of neem leaf extract on fertilizer nitrogen (N) transformation in soil have not been fully recognized. This study therefore aimed to evaluate the effects of five nitrification inhibitors on the status of soil inorganic N, urea hydrolysis, and nitrification: i) no inhibitor (control); ii) nitrapyrin; and three rates of neem leaf extract based on the dry weight of the raw material: iii) 1 g kg-1 soil; iv) 2 g kg-1 soil; v) 4 g kg-1 soil. Neem leaf extract in all rates increased urea hydrolysis rate on days 5-15. In contrast, nitrapyrin decreased urea hydrolysis on days 5-10, compared to the control. As for nitrification, neem leaf extract showed both stimulatory and inhibitory effects. The stimulation appeared on day 3, whereas inhibition occurred and peaked on days 5-15. Nitrapyrin showed inhibitory effects on days 10-15, reaching its peak on day 15. Increasing rates of neem leaf extract brought about increases in stimulation and inhibition of urea hydrolysis and nitrification. The results conclusively stated that the neem leaf extract had generally faster, stronger, and longer nitrification inhibition than nitrapyrin. The stimulation in the early phase and the inhibition in the later phase of the incubation of neem leaf extract were more pronounced with higher application rates.
Abbasi, M.K., Hina, M., Tahir, M.M., 2011. Effect of Azadirachta indica (neem), sodium thiosulphate and calcium chloride on changes in nitrogen transformations and inhibition of nitrification in soil incubated under laboratory conditions. Chemosphere 82(11), 1629–1635.
Allison, S.M., Prosser, J.I., 1991. Urease activity in neutrophilic autotrophic ammonia-oxidizing bacteria isolated from acid soils. Soil Biology and Biochemistry 23(1), 45–51.
Amato, M., Ladd, J.N., 1988. Assay for microbial biomass based on ninhydrin-reactive nitrogen in extracts of fumigated soils. Soil Biology and Biochemistry 20(1), 107–114.
Anderson, J.P.E., 1982. Soil respiration. [In] Page, A.L., Miller, R.H., Keeney, D.R. (Eds.), Agronomy monograph number 9, Part 2, Chemical and biological properties, 2nd ed. SSSA and ASA, Madison, WI, 831–871.
Arafat, S.M., El-Galil, A.A., Seeda, M.A., 1999. Improvement of nitrogen fertilizer efficiency with nitrification inhibitors in lowland rice. Pakistan Journal of Biological Sciences 2, 1184–1187.
Aspelin, V., Ekholm, J., 2017. Inhibition of nitrification in industrial wastewater– Identification of sources. Master of Science Thesis. Department of Chemical Engineering, Lund University, Lund, Sweden.
Bi, Q.-F., Chen, Q.-H., Yang, X.-R., Li, H., Zheng, B.-X., Zhou, W.-W., Liu, X.-X., Dai, P.-B., Li, K.-J., Lin, X.-Y., 2017. Effects of combined application of nitrogen fertilizer and biochar on the nitrification and ammonia oxidizers in an intensive vegetable soil. AMB Express 7(1), 198–198.
Bremner, J.M., Mulvaney, C.S., 1982. Nitrogen–Total. [In] Spark, D.L. (Ed.). Methods of soil analysis. Part 2. Chemical and microbiological propterties. SSSA Book Ser. 5. SSSA, Madison, WI, 595–624.
Gnanavelrajah, N., Kumaragamage, D., 1998. Effect of neem (Azadirachta indica a. Juss) materials on nitrification of applied urea in three selected soils of Sri Lanka. Tropical Agricultural Research 10, 61–73.
Guo, C., Wang, H., Zou, D., Wang, Y., Han, X., 2022. A novel amended nitrification inhibitor confers an enhanced suppression role in the nitrification of ammonium in soil. Journal of Soils and Sediments 22(3), 831–843.
Hasan, H.A.H., 2000. Ureolytic microorganisms and soil fertility: A review. Communications in Soil Science and Plant Analysis 31(15-16), 2565–2589.
He, H., Zhen, Y., Mi, T., Fu, L., Yu, Z., 2018. Ammonia-oxidizing archaea and bacteria differentially contribute to ammonia oxidation in sediments from adjacent waters of Rushan Bay, China. Frontiers in Microbiology 9, 116.
IPCC (Intergovernmental Panel on Climate Change), 2007. Climate Change 2007: Mitigation of climate change. Contribution of working group III to the fourth assessment report of the intergovernmental panel on climate change. Cambridge University Press, Cambridge.
Jiang, N.-J., Wang, Y.-J., Chu, J., Kawasaki, S., Tang, C.-S., Cheng, L., Du, Y.-J., et al., 2021. Bio-mediated soil improvement: An introspection into processes, materials, characterization and applications. Soil Use and Management 38, 69–93.
Kilani-Morakchi, S., Morakchi-Goudjil, H., Sifi, K., 2021. Azadirachtin-based insecticide: Overview, risk assessments, and future directions. Frontiers in Agronomy 3, 676208.
Kizilkaya, R., Samofalova, I., Mudrykh, N., Mİkaİlsoy, F., AkÇa, İ., Sushkova, S., Minkina, T., 2015. Assessing the impact of azadirachtin application to soil on ureaseactivity and its kinetic parameters. Turkish Journal of Agriculture and Forestry 39(6), 976–983.
Konwar, M.J., Borah, K., Rahman, W., Sutradhar, P., Goswami, G., 2016. A review on use of nitrification inhibitors for increasing nitrogen use efficiency. International Journal of Agricultural Science and Research 6(2), 107–118.
Kroetsch, D., Wang, C., 2008. Particle size distribution. [In] Carter, M.R., Gregorich, E.G. (Eds.), Soil sampling and methods of analysis. Canadian Society of Soil Science, CRC Press, and Taylor & Francis Group, Oxford, 713–725.
Kumar, D., Devakumar, C., Kumar, R., Das, A., Panneerselvam, P., Shivay, Y.S., 2010. Effect of neem-oil coated prilled urea with varying thickness of neem-oil coating and nitrogen rates on productivity and nitrogen-use efficiency of lowland irrigated rice under indo-gangetic plains. Journal of Plant Nutrition 33(13), 1939–1959.
Majumdar, D., 2005. Crop yield and soil nitrogen dynamics in an intermittently flooded rice field affected by nitrification inhibitors. Archives of Agronomy and Soil Science 51(6), 645–653.
Mohanty, S., Patra, A.K., Chhonkar, P.K., 2008. Neem (Azadirachta indica) seed kernel powder retards urease and nitrification activities in different soils at contrasting moisture and temperature regimes. Bioresource Technology 99(4), 894–899.
Mweetwa, A.M., Lubungo, A.C., Chishala, B.H., Phiri, M., 2016. Selected chemical properties, microbial activity and biomass of soils amended with aqueous neem leaf extract. Sustainable Agriculture Research 5(3), 103–112.
Nelson, D.W., Sommers, L.E., 1982. Total carbon, organic carbon, and organic matter. [In] Spark, D.L. (Ed.). Methods of soil analysis. Part 2. Chemical and microbiological propterties. SSSA Book Ser. 5. SSSA, Madison, WI, 539–579.
Orwa, C., Mutua, A., Kindt, R., Jamnadass, R., Anthony, S., 2009. Agroforestree database: A tree reference and selection guide version 4.0. World Agroforestry Centre, Kenya.
Pansu, M., Gautheyrou, J., 2006. Handbook of soil analysis: mineralogical, organic and inorganic methods. Springer-Verlag, Heidelberg.
Patra, D.D., Kiran, U., Chand, S., Anwar, M., 2009. Use of urea coated with natural products to inhibit urea hydrolysis and nitrification in soil. Biology and Fertility of Soils 45(6), 617–621.
Prasad, R., Power, J.F., 1995. Nitrification inhibitors for agriculture, health, and the environment. Advances in Agronomy 54, 233–281.
Ruanpan, W., Mala, T., 2016. The effect of some Thai medicinal herb extracts on nitrification inhibition. Modern Applied Science 10(2), 146–158.
Sabir, B.M., Gozal, A.S., Khudoykul, U.B.D., 2020. Biological nitrogen. Asian Journal of Multidimensional Research 9(3), 66-68.
Sahrawat, K.L., 1980. Control of urea hydrolysis and nitrification in soil by chemicals—Prospects and problems. Plant and Soil 57(2), 335–352.
Santhi, S.R., Palaniappan, S.P., Purushothaman, D., 1986. Influence of neem leaf on nitrification in a lowland rice soil. Plant and Soil 93(1), 133–135.
SAS Institute Inc., 2004. SAS/STAT® 9.1: User’s guide. Cary, NC, SAS Institute Inc.
Sharma, S.N., Prasad, R., 1995. Use of nitrification inhibitors (neem and DCD) to increase N efficiency in maize-wheat cropping system. Fertilizer Research 44, 169–175.
Sigurdarson, J.J., Svane, S., Karring, H., 2018. The molecular processes of urea hydrolysis in relation to ammonia emissions from agriculture. Reviews in Environmental Science and Bio/Technology 17(2), 241–258.
Solomon, M.G., B.Okon, P., Umoetok, S.B.A., 2008. Effects of neem extracts on soil properties, microbial populations and leaf area of fluted pumpkin (Telfairia occidentalis). Research Journal of Agronomy 2(1), 12–17.
Sparling, G.P., West, A.W., 1988. A direct extraction method to estimate soil microbial C: Calibration in situ using microbial respiration and 14C labelled cells. Soil Biology and Biochemistry 20(3), 337–343.
Sridharan, G.V., Hazarika, D., Bhat, M.R., Suraksha, R.S., Singh, S., 2017. Nitrification inhibition studies of neem coating on urea prills. Asian Journal of Chemistry 29(1), 196–198.
Stark, J.D., Walter, J.F., 1995. Persistence of azadirachtin A and B in soil: Effects of temperature and microbial activity. Journal of Environmental Science and Health, Part B 30(5), 685–698.
Stevenson, F.J., 1982. Nitrogen–Inorganic forms. [In] Spark, D.L. (Ed.). Methods of soil analysis. Part 2. Chemical and microbiological propterties. SSSA Book Ser. 5. SSSA, Madison, WI, 643-698.
Sun, H., Zhang, H., Powlson, D., Min, J., Shi, W., 2015. Rice production, nitrous oxide emission and ammonia volatilization as impacted by the nitrification inhibitor 2-chloro-6-(trichloromethyl)-pyridine. Field Crops Research 173, 1–7.
Svane, S., Sigurdarson, J.J., Finkenwirth, F., Eitinger, T., Karring, H., 2020. Inhibition of urease activity by different compounds provides insight into the modulation and association of bacterial nickel import and ureolysis. Scientific Reports 10(1), 8503.
Varel, V.H., 1997. Use of urease inhibitors to control nitrogen loss from livestock waste. Bioresource Technology 62(1), 11–17.
Vlek, P.L.G., Stumpe, J.M., Byrnes, B.H., 1980. Urease activity and inhibition in flooded soil systems. Fertilizer Research 1(3), 191–202.
Wilke, B.M., 2005. Determination of chemical and soil properties. [In] Margesin, R., Schinner, F. (Eds.), Manual for soil analysis – Monitoring and assessing soil bioremediation. Springer Verlag, Heidelberg, 47–95.
Wolt, J.D., 2000. Nitrapyrin behavior in soils and environmental considerations. Journal of Environmental Quality 29(2), 367–379.
Xi, R., Long, X.E., Huang, S., Yao, H., 2017. pH rather than nitrification and urease inhibitors determines the community of ammonia oxidizers in a vegetable soil. AMB Express 7(1), 129.
Yu, Y., Odindo, O., Xue, L., Yang, L., 2016. Influences of biochar addition on vegetable soil nitrogen balance and pH buffering capacity. IOP Conference Series: Earth and Environmental Science 41, 012029.
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