L-arginine application triggered soil hydrolytic activity
More details
Hide details
Department of Soil Science, College of Agriculture, Isfahan University of Technology, Iran
Submission date: 2022-08-23
Final revision date: 2022-12-09
Acceptance date: 2023-02-11
Online publication date: 2023-02-12
Publication date: 2023-05-19
Corresponding author
Sajedeh Khosrozadeh   

Department of Soil Science, College of Agriculture, Isfahan University of Technology, 84156-83111, Isfahan, Iran
Soil Sci. Ann., 2023, 74(1)161147
Application of amino acids to soils is reportedly associated with controversial responses in soil enzyme activities. The effects of L-arginine application on the fluorescein diacetate (FDA) hydrolysis and protease activity in an oak forest soil was investigated. The FDA hydrolysis and protease activity were regularly measured over a standard incubation period. The addition of L-arginine increased both FDA hydrolysis and protease activity after a lag time of 10 days. After 30 days, the ratio of FDA hydrolysis and protease activity in L-arginine-amended soil samples to those in the control reached 2.0 and 3.7, respectively. Moreover, FDA hydrolysis was found significantly (r = 0.67, P < 0.05) correlated with protease activity. It was concluded that L-arginine was able to stimulate FDA hydrolysis and protease activity, thereby making the soil hydrolytic system capable of facing more complicated substrates.
Adam, G., Duncan, H., 2001. Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils. Soil Biology and Biochemistry 33(7), 943-951.
Alef, K., Nannipieri, P., 1995. Enzyme activities, [In] K. Alef, P. Nannipieri (Eds.), Methods in Applied Soil Microbiology and Biochemistry. Academic Press, London, pp. 311-373.
Allison, S.D., Vitousek, P.M., 2005. Responses of extracellular enzymes to simple and complex nutrient inputs. Soil Biology and Biochemistry 37(5), 937-944.
Andersson, M., Kjøller, A., Struwe, S., 2004. Microbial enzyme activities in leaf litter, humus and mineral soil layers of European forests. Soil Biology and Biochemistry 36(10), 1527-1537.
Bremner, J.M., 1996. Nitrogen-Total, [In] D.L. Sparks, A.L. Page, P.A. Helmke, R.H. Loeppert (Eds.), Methods of soil analysis. Part 3. Chemical method-SSSA Book series no.5. Soil Science Society of America : American Society of Agronomy, Madison, USA, pp. 1085-1121.
Burt, R., 2004. Soil survey laboratory methods manual: soil survey investigation (Report No. 42, version 4.0), Department of Agriculture, Washangton, D.C; U.S.
Criquet, S., Tagger, S., Vogt, G., Le Petit, J., 2002. Endoglucanase and β-glycosidase activities in an evergreen oak litter: annual variation and regulating factors. Soil Biology and Biochemistry 34(8), 1111-1120.
Eilers, K.G., Lauber, C.L., Knight, R., Fierer, N., 2010. Shifts in bacterial community structure associated with inputs of low molecular weight carbon compounds to soil. Soil Biology and Biochemistry 42(6), 896-903.
Gaspar, M.L., Cabello, M.N., Pollero, R., Aon, M.A., 2001. Fluorescein Diacetate Hydrolysis as a Measure of Fungal Biomass in Soil. Current Microbiology 42(5), 339-344.
Geisseler, D., Horwath, W.R., 2008. Regulation of extracellular protease activity in soil in response to different sources and concentrations of nitrogen and carbon. Soil Biology and Biochemistry 40(12), 3040-3048.
Geisseler, D., Horwath, W.R., 2009. Relationship between carbon and nitrogen availability and extracellular enzyme activities in soil. Pedobiologia 53(1), 87-98.
Graham, M.H., Haynes, R.J., 2005. Organic matter accumulation and fertilizer-induced acidification interact to affect soil microbial and enzyme activity on a long-term sugarcane management experiment. Biology and Fertility of Soils 41(4), 249-256.
Green, V.S., Stott, D.E., Diack, M., 2006. Assay for fluorescein diacetate hydrolytic activity: Optimization for soil samples. Soil Biology and Biochemistry 38(4), 693-701.
Halsey, C.R., Lei, S., Wax, J.K., Lehman, M.K., Nuxoll, A.S., Steinke, L., Sadykov, M., Powers, R., Fey, P.D., 2017. Amino Acid Catabolism in Staphylococcus aureus and the Function of Carbon Catabolite Repression. mBio 8(1), e01434-01416.
Haynes, R.J., 1999. Size and activity of the soil microbial biomass under grass and arable management. Biology and Fertility of Soils 30(3), 210-216.
Jones, D.L., Healey, J.R., Willett, V.B., Farrar, J.F., Hodge, A., 2005. Dissolved organic nitrogen uptake by plants—an important N uptake pathway? Soil Biology and Biochemistry 37(3), 413-423.
Jones, D.L., Hughes, L.T., Murphy, D.V., Healey, J.R., 2008. Dissolved organic carbon and nitrogen dynamics in temperate coniferous forest plantations. European Journal of Soil Science 59(6), 1038-1048.
Lundgren, B., 1981. Fluorescein Diacetate as a Stain of Metabolically Active Bacteria in Soil. Oikos 36(1), 17-22. 10.2307/3544373.
McMahon, S., Schimel, J.P., 2017. Shifting patterns of microbial N-metabolism across seasons in upland Alaskan tundra soils. Soil Biology and Biochemistry 105(96-107.
Nannipieri, P., Muccini, L., Ciardi, C., 1983. Microbial biomass and enzyme activities: Production and persistence. Soil Biology and Biochemistry 15(6), 679-685.
Nourbakhsh, F., 2007. Decoupling of soil biological properties by deforestation. Agriculture, Ecosystems & Environment 121(4), 435-438.
Perucci, P., 1992. Enzyme activity and microbial biomass in a field soil amended with municipal refuse. Biology and Fertility of Soils 14(1), 54-60.
Renella, G., Szukics, U., Landi, L., Nannipieri, P., 2007. Quantitative assessment of hydrolase production and persistence in soil. Biology and Fertility of Soils 44(2), 321-329.
Sánchez-Monedero, M.A., Mondini, C., Cayuela, M.L., Roig, A., Contin, M., De Nobili, M., 2008. Fluorescein diacetate hydrolysis, respiration and microbial biomass in freshly amended soils. Biology and Fertility of Soils 44(6), 885-890.
SAS Institute, 2013. SAS Procedures Guide, Version 9.4. SAS Institute Inc., Cary, NC.
Schloter, M., Dilly, O., Munch, J.C., 2003. Indicators for evaluating soil quality. Agriculture, Ecosystems & Environment 98(1), 255-262.
Schnürer, J., Rosswall, T., 1982. Fluorescein Diacetate Hydrolysis as a Measure of Total Microbial Activity in Soil and Litter. Applied and Environmental Microbiology 43(6), 1256-1261.
Sharma, A.K., Singh, S.P., 2016. Effect of amino acids on the repression of alkaline protease synthesis in haloalkaliphilic Nocardiopsis dassonvillei. Biotechnology Reports 12(40-51.
Sims, G.K., Wander, M.M., 2002. Proteolytic activity under nitrogen or sulfur limitation. Applied Soil Ecology 19(3), 217-221.
Stubberfield, L.C.F., Shaw, P.J.A., 1990. A comparison of tetrazolium reduction and FDA hydrolysis with other measures of microbial activity. Journal of Microbiological Methods 12(3), 151-162.