Flood, i.e. temporary covering of the area by water and mass flow of water, can cause changes in the physicochemical properties of the soil, which are affected by it. Changes caused by flood can result in soil contamination, e.g. with heavy metals, as well as bacteriological and parasitological contamination. In connection with floods, there is a need to carry out monitoring works in the scope of changes in the physicochemical and biological properties of soils. The aim of the research was to assess the physicochemical and biological properties of soils of allotment gardens in Nysa after the flood in 2024, in terms of their current use and future development. The analyzed area affected by the flood in September 2024 is located in the area of allotment gardens in Nysa. The flood in Nysa was recorded on 14.09.2024, and the flood waters receded after a maximum of 14 days. The water in the allotment gardens stagnated for 7 to 14 days. In early spring 2025, 12 soil samples were taken from designated plots in two family allotment gardens. For physicochemical analyses, soil samples were taken from a depth of 0–20 cm, and for biological tests from a deeper layer, i.e. 15–30 cm, in accordance with the methodology in force at chemical-agricultural stations. The results of the research indicate that in most of the allotment garden soils were limed and at the same time disinfected. From a sanitary point of view, it is recommended to lime soils with a pH below 6.5. This treatment deacidifies the soil environment, but above all disinfects the soil. The soils of family allotment gardens in Nysa were characterized by a high and very high content of organic matter, which is necessary for growing plants sensitive to a lack of this substance in the soil. These soils were best supplied with assimilable phosphorus and magnesium, and the least with potassium. In order to provide plants with assimilable nutrients (P, K, Mg), while avoiding soil degradation, their abundance in these components should be maintained at least at an average level. The total content of macroelements in soils was within the average range for Polish arable soils. The studies show that the soils of allotment gardens in Nysa were characterized by low total potassium (K), sodium (Na) and sulfur (S) content. The content of zinc (Zn) and copper (Cu) in the tested soil samples was above the average value for arable soils. Higher contents of Zn and Cu in the tested soils indicate the use of agents enriched with these elements (e.g. plant protection products, such as copper sulfate, etc.). The soil samples analyzed did not exceed the permissible heavy metal content in accordance with the applicable Regulation on the method of assessing the contamination of the Earth's surface. The content of heavy metals in the analyzed soils did not show a negative impact on the ground environment of group II, which includes family allotment gardens. The assessment of the heavy metal content in the analyzed soils in relation to the IUNG-PIB guidelines showed that they are characterized by an increased content of Zn and Cu (level I). Microbiological contamination of greater significance occurred only in soils where basic treatments such as digging and liming were not applied. The presence of live eggs of intestinal parasites (Toxocara canis) in only two samples of the analyzed soils was not necessarily related to last year's flood, but may result from accidental contamination with feces, because the carriers of these parasites are wild animals (e.g. foxes, dogs, cats, etc.). The soil of allotment gardens in Nysa should not be disqualified from horticultural and recreational use. Due to the increased content of Zn and Cu in the soil, it is recommended to grow vegetables with the exclusion of consumption by children and infants.