The biochemical characteristics of phosphate bacteria capable of increasing soil phosphorus bioavailability in Andisols
Jenderal Soedirman University, Agriculture Faculty, Agrotechnology Department dr. Soeparno Street Purwokerto 53123, Central of Java, Indonesia
Data publikacji online: 03-06-2020
Data publikacji: 03-06-2020
Data nadesłania: 05-11-2019
Data akceptacji: 04-03-2020
Soil Sci. Ann., 2020, 71(2), 125–132
N-Acyl Homoserine Lactone (N-AHL) has been known as the quorum sensing (QS) signals that control phosphate bacteria (PB) activities in enhancing soil phosphorus (P) availability. This study was aimed at determining: 1) the bacteria biochemical characteristics capable of soil P solubilizing, 2) the N-AHL production types and 3) the best plant roots extract as a N-AHL source. The PB species were determined using 16S rRNA analysis. The determination of the organic acids, phosphatase, phytase, N-AHL and dissolved P was carried out using HPLC, para-Nitro Phenyl Phosphate (pNPP), Na-phytate and spectrophotometry methods, respectively. The PB isolates obtained were classified as Pseudomonas trivialis, P. putida and P. fluorescens. The PB secreted citric, lactic, malonic, oxalic and acetic acids amounting to 156.25 mg kg-1. The PB phosphatase and phytase activities ranged from 12 to 47 mg PO43- dm-3 h-1. The P solubilized in the PB Pikovskaya inoculated was greater with the amounts between 147.66 and 194.61 mg P kg-1 as control compared (31.06 mg P kg-1). The PB inoculation also produced greater mineralized organic P (63.69 mg P kg-1) than the control (23.7 mg P kg-1). The PB secreted N-AHL greatest was butanoyl-AHL (C4-AHL). The 30% of root extract corn seemed to be the best source of N-AHL as it could increase P dissolution even 300% in comparison with control variant.
Abbasi, M.K., Musa, N., Manzoor, M., 2015. Mineralization of soluble P fertilizers and insoluble rock phosphate in response to phosphate-solubilizing bacteria and poultry manure and their effect on the growth and P utilization efficiency of Chilli (Capsicum annuum L.). Biogeosciences 12, 4607–4619.
Azeem, M., Riaz, A., Chaudhary, A.N., Hayat, R., Hussain, Q., Tahir, M.I., Imran, M., 2014. Microbial phytase activity and their role in organic P mineralization. Archives of Agronomy and Soil Science 14, 1–16.
Badura, L., 2004. Biodiversity and its role in the functioning of ecosystems. Soil Science Annual 40(1), 321–335. ctioning-of-ecosystems.
Banach A., Wolińska A., Błaszczyk M., Stępniewska Z., 2015. The influence of soil properties and land use on the phosphate level in soils from Lubelskie region. Polish Journal of Agronomy 22, 3–9.
Barriuso, J., 2015. Quorum sensing mechanisms in fungi. AIMS Microbiology 1(1), 37–47.
Belgaroui, N., Berthomieu, P., Rouached, H., Hanin, M., 2016. Secretion of bacterial phytase PHY-US417 by arabidopsis roots reveals its potential for increasing phosphate acquisition and biomass production during co-growth. Plant Biotechnology Journal 14, 1914–1924.
Brogowski, Z., Kwasowski, W., 2009. Phosphorus adsorption by organic and mineral parts of soil. Soil Science Annual 40(1), 12–21.
Castagno, L.N., Estrella, M.Z., Sannazarro, A.I., Grassano, A.E., Ruiz, Q.A., 2011. Phosphate-solubilization mechanism and in vitro plant growth promoting activity mediumted by Pantoea eucalypti isolated from Lotus tenuis rhizosphere in the salado river basin (Argentina). Journal of Applied Microbiology 110, 1–15. 2011.04968.x.
Dangjarean, H., Tantachasatid, P., Jitaksorn, S., Sadowsky, M.J., Sajjaphan, K., 2015. Plant growth-promoting ability and N-acyl-homoserine lactones production by siderophore-producing rhizobacteria. Kasetsart Journal (Natural Science) 49, 573–582. https://
Delfim, J., Schoebitz, M., Paulino, L., Hirzel, J., Zagal, E., 2018. Phosphorus availability in wheat, in volcanic soils inoculated with phosphate-solubilizing. Bacillus thuringiensis. Sustainability 10(1), 144–159.
Gao, L., Kong, F., Feng C., Wang, J., Gao, J., Shen, G., Zhang, C., 2016. Isolation, charac-terization and growth promotion of phosphate-solubilizing bacteria associated with Nicotiana tabacum (Tobacco). Polish Journal of Environmental Studies 25(3), 993 –1003.
Götz-Rösch, C., Sieper, T., Fekete, A., Schmitt-Kopplin, P., Hartmann, A., Schröder, P., 2015. Influence of bacterial N-Acyl Homoserine Lactones on growth parameters, pigments, antioxidative capacities and the xenobiotic phase II detoxification enzymes in barley and yam bean. Frontier Plant Science 6 (205), 1–13.
Janda, J.M., Abbott, S. L., 2007. 16S rRNA gene sequencing for bacteria identification in the diagnostic laboratory: pluses, perils, and pitfalls. Journal of Clinical Microbiology 45(9), 2761–2764.
Kobus, J., 1999. Interaction between soil, plant, and microorganisms. Soil Science Annual 50(3), 89–110.
Kumar, S., Nei, M., Dudley, J., Tamura, K., 2008. MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences. Briefings in Bioinformatics 9, 299–306.
Liu, N., Yu, M., Zhao, Y., Cheng, J., An, K., Zhang, X.H., 2017. PfmA, a novel quorum-quenching N-Acyl Homoserine Lactone acylase from Pseudoalteromonas flavipulchra. Microbiology 163, 1389–1398.
Ma, Z.P., Lao, Y.M., Jin, H., Lin, G.H., Cai, Z.H., Zhou, J., 2016. Diverse profiles of AI-1 type quorum sensing molecules in cultivable bacteria from the mangrove (Kandelia obovata) rhizosphere environment. Frontiers in Microbiology 7, 1–14.
Mahmoudi, E., 2015. Signaling molecules from Lactuca Sativa L. induced quorum sensing phenotypes in bacteria. Journal of Plant Protection Research 55(2), 166–171.
Palmer, A.G., Senechal, A.C., Mukherjee, A., , J.M., Blackwell, H.W. 2014. Plant responses to bacterial N-Acyl Homoserine Lactones are dependent on enzymatic degradation to homoserine. ACS Chemical Biology 9(8), 1834–1845.
Patki, J.M., Singhand, S., Mehta, S., 2015. Partial purification and characterization of phytase from bacteria inhabiting the mangroves of the western coast of India. International Journal of Current Microbiology and Applied Sciences 4(9), 156–169.
Pikovskaya, R.I., 1948. Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiology 17, 362–370. sjt1aadkposzje))/reference/ReferencesPapers.aspx?ReferenceID=1514468.
Rani, S., Kumar, A., Malik, A.K., Koplin, P.A., 2011. Occurrence of N-acyl homoserine lactones in extracts of bacterial strain of Pseudomonas aeruginosa and sputum sample evaluated by gas chromatography-mass spectrometry. American Journal of Analytical Chemistry 2, 294–302. https://doi:10.4236/ajac.2011.....
Ransome, E., Munn, C.B., Halliday, N., Amara, M.C., Tait, K., 2013. Diverse profiles of N-Acyl Homoserine Lactone molecules found in cnidarians. FEMS Microbiology Ecology 2013, 1–15.
Rathi, M., Gaur, N., 2016. Phosphate solubilizing bacteria as biofertilizer and its applications. Journal of Pharmacy Research 10(3), 146–148.
Stella, M., Syahren A.M., 2016. Application of phosphate solubilising microorganisms to increase the solubilisation of rock phosphates in soil. Journal of Tropical Agriculture and Food Science 44(1), 9–18. Application_of_phosphate_solubilising_microorganisms_to_increase_the_solubilisation_of_rock_phosphates_in_soil.
Saeid, A., Prochownik, E., Dobrowolska-Iwanek, J., 2018. Phosphorus solubilization by bacillus species. Molecules 23, 1–18. https://doi/10.3390/molecules2....
Tan, P.W., Tan, W.S., Yunos, N.Y.M., Mohamad, N.I., Adrian, T.G.S., Yin, W.F., Chan, K.G., 2014. Short chain N-acyl homoserine lactone production in tropical marine Vibrio sinaloensis strain T47. Sensors 14(7), 12958–12967. s140712958.
Villamizar, G.A.C., Nacke, H., Griese, L., Tabernero, L., Funkner, K, Daniel, R., 2019. Characteristics of the first protein tyrosine phosphatase with phytase activity from a soil metagenome. Genes 10, 1– 6.
Weiland-Bräuer, N., Pinnow, N., Schmitz, R.A. 2015. Novel reporter for identification of interference with acyl homoserine lactone and autoinducer-2 quorum sensing. Applied and Environmental Microbiology 81(4), 1477–1489.
Weimin, C., Yang, F., Zhang, L., Wang, J., 2016. Organic acid secretion and phosphate solubilizing efficiency of Pseudomonas sp. psb12: effects of phosphorus forms and carbon sources. Geomicrobiology Journal 33(10), 870–877. 01490451. 2015.1123329.
Wong, C.S., Koh, C.L., Sam, C.K., Chen, J.W., Chong, Y.M., Yin, W.F., Chan, K.G., 2013. Degradation of bacterial quorum sensing signaling molecules by the microscopic yeast Trichosporon loubieri isolated from tropical wetland waters. Sensors 13, 12943–12957.
Yuquan, W., Zhao, Y., Shi, M., Cao, Z., Lu, Q., Yang, T., Fan, Y., Wei, Z., 2018. Effect of organic acids production and bacterial community on the possible mechanism of phosphorus solubilization during composting with enriched phosphate-solubilizing bacteria inoculation. Bioresource Technology 247, 190–199. j.biortech.2017.09.092.
Zhang, C., Zhu, S., Jatt, A.B., Zeng, M., 2016. Characterization of N-Acyl Homoserine Lactones (AHLs) producing bacteria isolated from vacuum-packaged refrigerated turbot (Scophthalmus maximus) and possible influence of exogenous AHLs on bacterial phenotyp. Journal of General and Applied Microbiology 62, 60–67. 10.2323/jgam.62.60.